Results: Our findings show that neotame causes intestinal epithelial cell apoptosis and death with siRNA knockdown of T1R3 expression significantly attenuating the neotame-induced loss to cell viability. Similarly, neotame exposure results in barrier disruption with enhanced monolayer leak and reduced claudin-3 cell surface expression through a T1R3-dependent pathway. Using the gut bacteria models, E. coli and E. faecalis, neotame significantly increased biofilm formation and metabolites of E. coli, but not E. faecalis, reduced Caco-2 cell viability. In co-culture studies, neotame exposure increased adhesion capacity of E. coli and E. faecalis onto Caco-2 cells and invasion capacity of E. coli. Neotame-induced biofilm formation, E.coli-specific Caco-2 cell death, adhesion and invasion was identified to be meditated through a taste-dependent pathway.

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This type of product can also be used pre- and post-workout, and in fact, the directions suggest taking up to two scoops a day, but one scoop at separate times. Again, for me, I like to sip something like this during my workout and do other things pre- and post-workout, but either way, this type of supplement is quite flexible.

The one I’m reviewing is the Miami Vice flavor. When you open the canister, there’s not much of a scent, which is actually a good thing. I’m always suspicious when I’m overpowered by flavoring odors. This mildness transfers over to the taste. The one level scoop mixes easy in either 8- or 16-ounces of water (I prefer more the former than the latter), and even if in just 8 ounces of water you’re hit with mild tropical flavors, with hints of coconut and pineapple standing out the most in the flavor profile. The scoop also has an extra-long handle for reaching down the bottom of the canister, although this can be a bit of an annoyance when trying to reclose the canister with more of the product still in it.

Aminogex Ultra is vegan and is sweetened with sucralose. The calories are negligible for something like this, but the nutritional profile is high. The estimate is about 30 scoops/servings per container.

Overall, I appreciate all the nutrition that’s packed into this product in well-thought-out ratios. I also appreciate the mild flavor profile. Versus some other products in this category that overpower you with sweetness, there’s more subtleness to the blend, although as with many of these products, the flavor profile builds the more you drink the serving. If you’re looking for a good EAA/BCAA blend, this is a great choice.

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As always, it’s the taste that matters with these things. The Homemade Vanilla is a bit on the thin side, but does have a smooth, creamy mouth feel. It definitely tastes like vanilla, although it does have a slightly metallic aftertaste. It’s not my favorite flavor, but it’s something I could certainly drink in a pinch.

I personally tend to like more calories in my protein shakes (like with my preferred Labrada Lean Body) because I like to use it as a breakfast. With that said, so few calories for such relatively high protein is really impressive, so these would make for an ideal snack. Even if you’re not a diabetic, it’s easy to recommend these protein shakes.

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